replication 1 of SpM10 - SRA

SRX26846376: replication 1 of SpM10
1 ILLUMINA (Illumina HiSeq 2500) run: 25.7M spots, 7.7G bases, 2.7Gb downloads

Design: After the total RNA of male and female flowers was extracted by TRIzol reagent (Life technologies), mRNA was enriched by Oligo (dT) beads. The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA with random primers. Second-strand cDNA was synthesized by DNA polymerase I, RNase H, dNTP, and buffer. The cDNA fragments were then purified with QiaQuick PCR extraction kit, end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, PCR amplified,and sequenced using Illumina HiSeq? 2515 by Gene Denovo Biotechnology Co..

Submitted by: Henan Normal University

Study: Full-length transcriptome sequencing of spinach

PRJNA607896 • SRP250242 • All experiments • All runs

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The study goal is to uncover the exact full-length of mRNA, alternative splicing events, alternative polyadenylation evens, SNP, fusion and long non-coding RNA, etc., which increased the diversity of spinach transcriptome. This work will provide voluable information for functional research in spianch and related plants.

Sample:

SAMN24858646 • SRS11624124 • All experiments • All runs

Organism: Spinacia oleracea

Library:

Name: SpM10-1

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 25.7M spots, 7.7G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR31477783	25,728,825	7.7G	2.7Gb	2024-11-23

ID:: 36269886

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